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INDUSTRIAL HEALTH 1990,28,21-30
Antinucleolar Autoantibody Induced in Mice
by Mercuric Chloride
Junzo SAEGUSA1), Shigeki YAMAMOTO2),
Hiroshi IWAI2) and Katsumoto UEDA2)
1) National Institute of Industrial Health
21-1, Nagao 6-chome, Tama-ku, Kawasaki 214 Japan
2) Department of Veterinary Public Health, The Institute of Public Health
4-6-1, Shiroganedai, Minato-ku, Tokyo 108, Japan.
(Received Decembre 6, 1989 and in revised form December 28, 1989)
Abstract: Mercuric chloride (MC) was deministered to mice subcutaneously 3 times a week for 4 weeks. When inbred BALB/c, CBA/JN, C3H/He, C57BL/6 and DBA/2 and outbred ICR faamale mice were given 1 mg/kg MC, 8 of 10 ICR mice developed antinucleolar autoantibodies (AnuA) of the IgG class but none fo the inbred mice produced AnuA. So the ICR mice were used in the following experiments. In a kinetic study, in which mice were given 1.5 mg/kg MC, the positivities and titers of AnuA increased time-dependently, and were kept at high levels until 8 weeks after the final treatment. The AnuA titers of mice receiving 0.5-2.0 mg/kg increased dose-dependently, while the positivity was similar at all dosages. The antibody titers were higher in mice pretreated with an intramuscular injection of either complete or incomplete Freund's adjuvant. The titer increase was more prominent in mice administered doses lower that 0.5 mg/kg. Neither nude mice nor mice treated with a monoclonal antibody to L3T4 (GK1.5) developed AunA. These results indicate that the induction of AnuA by MC depend primarily on L3T4-positive T cells.
Key words: Mercuric chloride-Antinucleolar autoantibody-T cells-L3T4
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